【評(píng)價(jià)原理】
色斑的形成與皮膚中黑色素的沉著有關(guān)，祛斑功效實(shí)質(zhì)上是對(duì)黑色素的清除作用。通過(guò)斑馬魚(yú)技術(shù)能簡(jiǎn)潔而有效的評(píng)價(jià)化妝品的祛斑功效。
斑馬魚(yú)在發(fā)育初期全身透明，胚胎發(fā)育至 24 h時(shí)黑色素開(kāi)始從視網(wǎng)膜上皮生長(zhǎng)，48h逐漸形成穩(wěn)定并可見(jiàn)的黑色素色斑。將受試物于斑馬魚(yú)發(fā)育48h時(shí)加入魚(yú)水中，72h時(shí)觀察黑色素的降低程度，從而評(píng)價(jià)祛斑功效。
【實(shí)驗(yàn)方案】
斑馬魚(yú)分成若干組，含正常對(duì)照組和供試品組。正常對(duì)照組不做處理，供試品組使用受試化妝品，孵育一段時(shí)間后，對(duì)斑馬魚(yú)頭部進(jìn)行拍照，利用高級(jí)圖像處理軟件分析斑馬魚(yú)頭部的黑色素信號(hào)強(qiáng)度，從而評(píng)價(jià)其祛斑功效。
【評(píng)價(jià)指標(biāo)】
皮膚黑色素信號(hào)強(qiáng)度
【結(jié)果展示】（展示圖片僅供參考，實(shí)際實(shí)驗(yàn)組別依據(jù)合同而定）
圖1. 斑馬魚(yú)頭部黑色素表型圖（藍(lán)色為分析區(qū)域）
【評(píng)價(jià)結(jié)論】
1、經(jīng)過(guò)各組斑馬魚(yú)對(duì)比實(shí)驗(yàn)，陽(yáng)性對(duì)照組和化妝品組與正常對(duì)照組對(duì)比，斑馬魚(yú)頭部只有少量的黑色素。
2、本實(shí)驗(yàn)證實(shí)了該化妝品具有祛斑作用。
【參考文獻(xiàn)】
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